Validating Real-Time Polymerase Chain Reaction (PCR) Assays
Generally, in qPCR assays an r 2 of not less than 0.99 is considered acceptable. The linearity and reportable range should be carried out on at least 5 log-dilutions of the target nucleic acid extracted from an appropriate sample type (serum, urine, NPA etc.,) in triplicate, ideally on two different thermocyclers.
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